Dextran has been used in immobilization in biosensors. Pdf dextran is a complex, branched glucose polymer carbohydrate with variable glycoside linkages and. Effects of cultural conditions on dextran production by. Using the spectrophotometric method, as a new method, the influence of dextran on the sucrose solubility and metastable zone width has been studied.
Specimens of pamylase limit dextrin, soluble starch, amylopectin, native b512 dextran, and a commercial preparation of low about 80,000 molecular weight clinical dextran did not affect reaction rate or oligosaccharide production appre. Production of dextran from free and immobilized cells of leuconostoc mesenteroides kibge ha1 using 10% sucrose in acrylamide. It is also used in some sizeexclusion chromatography matrices. Pharmacosmos launches new website on novel cryoprotectant pentaisomaltose as a step towards the commercialisation of the novel dmsofree cryoprotectant pentaisomaltose the website. Dextran can be used as a stabilizing coating to protect metal nanoparticles from oxidation and improve biocompatibility. Dextran may also be used for purposes not listed in this medication guide. Factors injhencing dextran production numerous factors, only a few of which have been studied 1,4, appear to influence the properties and the. You have free access to this content a study of dextran production from maltodextrin by cell suspensions of gluconobacter oxydans ncib 4943. Pdf fermentative production of dextran using food industry. Dextran production is always accompanied by accumulation of fructose in the. Production of dextransucrase, dextran and fructose from. The formation of dextran in wine was shown by pasteur to be due to the activity of micro. Effect of incubation time on dextran production the effect of incubation time on dextran production by the leuconostoc spp was done as described by sarwat et al.
The molecular weight distribution of the resulting production was less disperse than when clinical dextran. Pharmacosmos has for a number of years worked on a carbohydratebased alternative to dmso for cryopreservation this has led to the development of pentaisomaltose, which holds significant promise to enable dmsofree cryopreservation of a number of cell types, including among other hematopoetic stemcells, cord blood, and t cells. Nov 01, 2016 read production and structural characterisation of dextran from an indigenous strain of leuconostoc mesenteroides ba08 in whey, international journal of dairy technology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. This plant has the capacity to simultaneously produce both 10% and 20% iron dextran at the rate of up to 11,000 liters per week, and 250 kilograms per month of dextran sulphate. The former strains convert sucrose into dextran with the dextransucrase enzyme whereas the latter convert maltodextrins into. Effect of ph on dextran production is shown in fig. Ls1 and li1 was found to be 15% and 25% respectively. And technology abstract in the present study, the production of dextran from date extract. Production of high viscosity dextranthe viscosity of dextran from. Regulations food and drug administration fda iron dextran complex is a prescription drug subject to labeling and other requirements. Method for the production of dextran comprising the following steps. Dextran was discovered by louis pasteur as a bacterial product in wine but mass production was only possible after the development by allene jeanes of a process using bacteria.
To enhance the yield of dextran, effects of various parameters such as sucrose concentration, ph, temperature, incubation and inoculum percentage were analyzed. Pharmaceutical quality dextran pharmacosmos dextran. Found in the wild, but for the commercial production to receive seed, rs 0 per piglet to. The global leader in pharmaceutical dextran pharmacosmos. However, when these conditions were controlled, dextrans of high or of low viscosity were obtained as desired.
On the mechanism of dextran formation certain bacteria. Pdf fermentative production of dextran using food industry wastes. Hehre in 1941 reported the first cellfree synthesis of dextran using sucrose as. On the production of dextran by free and immobilized dextransucrase. Read production and structural characterisation of dextran from an indigenous strain of leuconostoc mesenteroides ba08 in whey, international journal of dairy technology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at. Dextran is a complicating contaminant in the refining of sugar because it elevates the viscosity of sucrose solutions and fouls plumbing. Characterization of the dextran produced by leuconostoc mesenteroides from date fruit extract m m. Structure the dextran elaborated by leuconostoc mesenteroides nrrl b512f consists of an a1 6linked glucan with side chains attached to the 3positions of the backbone glucose units. Nazemi 1 1department of food science and technology, college of agriculture, shiraz university, shiraz, i.
Small family 5 to 20 members, it was placed in the endangered category according to iucn in 1960. Historically, dextrans had been long recognized as contaminants in sugar processing and other food production. On the basis of high enzyme activity a newly isolated strain of l. Clinical dextran was synthesized when the polysaccharides produced in the presence of maltose were used as acceptor of a second synthesis reaction. In agreement with the literature the experimental data show that the dextran has a negligible effect on the sucrose solubility. Dextran has been described for use in the production of foodstuffs. Structure, physicalchemical properties and reactivity 2. Introduction a brief search of the chemical abstracts database for dextran and dextran related titles leaves one in little doubt of the prolific interest in this area. These experiments were performed to gain an insight into the time span needed to examine dextran production from g.
Characterization of the dextran produced by leuconostoc. Production of high viscosity dextran the viscosity of dextran from. Singleuse technology in production of dnafree pcr reagents background for over 20 years, molecular research has been a driving force behind advances in health care. Dextran is now synthesized from sucrose by certain lactic acid bacteria, the bestknown being leucon stoc meteoroids and streptococcus mutants. Morphological, biochemical, and 16s rrna sequencing analysis identified the strain as leuconostoc mesenteroides. Incubation was done at 2, 4, 6, 8, 12, 16, 20, 24, 36, and 48 h after which dextran. Dextrans can be formed by many microorganisms and are not welldefined substances with specific properties. Needleless electrospinning concerns selforganization of polymeric jets on free liquid surfaces and is a result of electrohydrodynamics in the liquid surface 19. Production and structural characterisation of dextran from an. A novel process for the production of clinical dextran a dissertation. It was probably the first biopolymer produced on an industrial scale by fermentation when production started around 1948.
Statistical optimization of media for dextran production. Abstract dextransucrase from leuconostoc mesenteroides was produced in a semicontinuous culture with slow addition of a concentrated sucrose solution. Dextran is the name given to a large class of extracellular bacterial polysaccharides composed almost exclusively of glucose units linked predominantly by 1. Reliable figures for the annual world production of dextran are not available but. May 22, 2019 dextran 40 pharmacokinetics absorption onset. Jul 03, 2018 infed iron dextran injection usp is a dark brown, slightly viscous sterile liquid complex of ferric hydroxide and dextran for intravenous or intramuscular use.
Aug 02, 2017 dextran has been used in immobilization in biosensors. White paper dna free pcr reagents singleuse technology in production of dna free pcr reagents background for over 20 years, molecular research has been a driving force behind advances in health care. Guangxi nanning taoyuan veterinary drugs factory api active pharmaceutical ingredient iron dextrose,veterinary injections,powder,premix,iron dextran,animal pharmaceuticals,api dextran,api chemical,iron dextran solution,iron injection,10ml 10% iron dextran,10ml 15% dextran,100ml dextran,blood system agents,iron dexdran. Dextran nanofiber production by needleless electrospinning. When dextran is used in a pharmaceutical product or medical device, a pharmaceutical quality of dextran should be applied. Any condition that has a confirmed nucleic acid target e.
The production of dextran has been extensively studied in lab of genera, viz. Pdf production and characterization of dextran from leuconostoc. Dextran net charge and fixability we employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. This dissertation is brought to you for free and open access by the graduate school at lsu digital commons. Dextran and its derivatives can also be used as adjuvants, emulsifiers, carriers and stabilizers in pharmaceutical and cosmetic preparations. Production and structural characterisation of dextran from. Continuous production of dextran from immobilized cells of. Exposure during production may be sitelimited, because only one manufacturer of iron dextran was identified in the united states in 2009 sri 2009. Fermentative production of dextran using leuconostoc spp. Dextran is used in the osmotic stress technique for applying osmotic pressure to biological molecules. A sequential statistical methodology comprising of plackettburman and response surface methodology rsm were applied. Co, for its formation and in older cultures it may also be found free in solution. Any of a group of branched polysaccharides with various molecular weights that are used to prevent thrombosis, as plasma volume expanders, and as food. A quantitative study of the production of dextran from sucrose by.
Dextran has also been used in bead form to aid in bioreactor applications. Use of the information, documents and data from the echa website is subject to the terms and conditions of this legal notice, and subject to other binding limitations provided for under applicable law, the information, documents and data made available on the echa website may be reproduced, distributed andor used, totally or in part, for noncommercial purposes provided that echa is. Pdf dextran is a dglucose polymer which is produced by leuconostoc. Kinetic studies are a vital part of the overall investiga tion of product. Iron dextran complex canada sri 2009 and is available. Immobilized dextransucrase looks promising for low. Institute of sustainable halophytes utilization, university of karachi75270, pakistan 3. Pdf dextran is a dglucose polymer which is produced by leuconostoc mesenteroides grown in a sucroserich media. Therefore, this paper aimed to fill the current knowledge gap through studying dextran nanofibrous layer production by the needleless electrospinning method. Dextransucrase ds is involved in dextran production in the presence of a rich carbon source. The results also show that this impurity decreases the sucrose metastable zone width. Centre for molecular genetics, university of karachi, pakistan 2. Nov 18, 2019 dextran is used to treat hypovolemia decreased volume of circulating blood plasma, that can result from surgery, trauma or injury, severe burns, or other causes of bleeding. A novel process for the production of clinical dextran.
The optimum sucrose concentration for the leuconostoc spp. Factors injhencing dextran productionnumerous factors, only a few of which have been studied 1,4, appear to influence the properties and the. Infed iron dextran injection usp is a dark brown, slightly viscous sterile liquid complex of ferric hydroxide and dextran for intravenous or intramuscular use each ml contains the equivalent of 50 mg of elemental iron as an iron dextran complex, approximately 0. A novel process for the production of clinical dextran a dissertation submitted to the graduate faculty of the louisiana state university and agricultural and mechanical college in partial fulfillment of the requirements for the degree of doctor of philosophy in the department of microbiology by doman kim b. A process for preparing by means of microorganisms or enzymes, dextrane having a molecular weight suitable for clinical use starting from a saccharosecontaining substrate, wherein it is carried out continuously with continuous supply of substrate and microorganisms or enzymes and continuous discharge of dextrancontaining reaction mixture from which the dextran is then recovered. Dextran is used to treat hypovolemia decreased volume of circulating blood plasma, that can result from surgery, trauma or injury, severe burns, or other causes of bleeding. Iv, issue 4 july 2016 3965 classification and chemical structure of dextran hehre in 1941 reported the first cell free synthesis of dextran using sucrose as the substrate from enzyme dextransucrase. On the production of dextran by free and immobilized dextransucrase on the production of dextran by free and immobilized dextransucrase monsan, p lopez, a. Production of high viscosity dextran the viscosity of dextran from leuconostoc mesenteroides nrrl b512 was found to be influenced greatly by cultural conditions. Dextran was discovered by louis pasteur as a microbial product in wine, but mass production was only possible after the development by allene jeanes of a process using bacteria. A short article about dextran and its effects on the sugar industry rafrogblogus.
The formation of dextran in wine was shown by pasteur to be due to the activity of microbes. Present study reports statistical media optimization for dextran production for lactic acid bacteria, isolated from indian traditional fermented idli batter. Ls1and li1 isolated from sauerkraut and idli batter was selected for dextran production. Current production is approximately 8,000 liters of iron dextran per week and approximately 250 kilos of dextran sulphate per quarter.
Maximum plasma volume reached within several minutes after the end of the infusion. Dextran definition of dextran by the free dictionary. Ep0087404a2 a process for the production of dextran. Farwa sarwat 1, shah ali ul qader 2, afsheen aman 3, nuzhat ahmed 1. All pharmaceutical quality dextran manufactured by pharmacosmos meets the quality standards of the pharmacopoeia products dextran 1, 40, 60, and 70. Extent and duration of the expansion in plasma volume vary with the volume infused, preadministration plasma volume, and the rate of renal clearance. On the production of dextran by free and immobilized. A study of dextran production from maltodextrin by cell. Inert dextran and its derivatives are widely used in biological and biochemical research.
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